anti mouse cd8 pe cy7  (Cell Signaling Technology Inc)

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Antibodies used for flow cytometry analysis and sorting of T cell populations from tumor and control bladder tissues.

Article Snippet: PE-Cy7 Mouse Anti-Human CD8 , RPA-T8 , BD biosciences.

Techniques: Flow Cytometry, Staining

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Gating strategy for analysis of Tregs, Th cells, and CD8 + T cells ( A ). Immune cell infiltration measured as CD45 + out of all viable cells ( B ). Frequency of T cells and T cell subsets out of all CD45 + cells ( C – G ). Treg = regulatory T cells, Th = T helper, NMIBC = non-muscle-invasive bladder cancer, MIBC = muscle-invasive bladder cancer. Statistical significance was determined by the Kruskal–Wallis test followed by the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: PE-Cy7 Mouse Anti-Human CD8 , RPA-T8 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Infiltration of CD45 + immune cells out of all viable cells and frequency of T cell populations out of CD45 + immune cells in tumor samples according to sex ( A – D , F , G ). Ratio of CD4 + /CD8 + T cells ( E ) Statistical significance was determined by Kruskal–Wallis test followed by two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: PE-Cy7 Mouse Anti-Human CD8 , RPA-T8 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Frequency of immune cells and T cell populations in tumor samples classified according to the Lund Taxonomy molecular subtypes. Infiltration of CD45 + immune cells among all viable cells ( A ). Immune infiltration in MIBC samples only ( B ). Percentages of T cells, CD4 + T cells, and CD8 + T cells out of all CD45 + cells ( C – E ). Ratio of CD4 + /CD8 + T cells ( F ). Percentages of Tregs and Th cells out of CD45 + cells ( G , H ). Statistical significance was determined through the Kruskal–Wallis test followed by the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: PE-Cy7 Mouse Anti-Human CD8 , RPA-T8 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: T cell populations in tumor samples classified according to the Lund Taxonomy molecular subtypes. Percentage of T cells ( A ), CD8 + T cells ( B ), CD4 + T cells ( C ), Tregs ( D ), and Th ( E ) out of all viable cells. Statistical significance was determined by Kruskal–Wallis test followed by two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli post-test.

Article Snippet: PE-Cy7 Mouse Anti-Human CD8 , RPA-T8 , BD biosciences.

Techniques:

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Transcriptional analysis of CD8 + T cells from non-malignant bladder tissue and from GU and Uro tumor biopsies. Principal component analysis ( A ). Heatmap of differentially expressed genes between CD8 + T cells from GU and Uro subtypes ( B ). GSEA using an exhaustion profile (Tirosh et al.’s 28-gene exhaustion profile) ( C ). Volcano plot with the differentially expressed genes (GU vs. control) marked in red, with the top 40 annotated ( D ). Volcano plot with the differentially expressed genes (Uro vs. control) marked in red, with the top 40 annotated ( E ). Percentage of CD8 + cells expressing the exhaustion markers CTLA-4, LAG3, PD-1, TIGIT, or TIM-3 measured by flow cytometry ( F ). Statistical significance assessed by Mann–Whitney U test.

Article Snippet: PE-Cy7 Mouse Anti-Human CD8 , RPA-T8 , BD biosciences.

Techniques: Expressing, Flow Cytometry, MANN-WHITNEY

Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Pathway analysis of differentially expressed genes between CD8 + T cells from GU subtype and the control ( A ) and Uro subtype and the controls ( B ). padj = 0.05 marked in red.

Article Snippet: PE-Cy7 Mouse Anti-Human CD8 , RPA-T8 , BD biosciences.

Techniques:

Journal: bioRxiv

Article Title: Assessing a Role for Sarcospan in Immune Function

doi: 10.1101/2024.03.31.587501

Figure Lengend Snippet: Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and CD8+ T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.

Article Snippet: Cells were incubated with anti-mouse primary antibodies to assess immune populations with the following conjugated antibodies: B220-APC (Tonbo Biosciences, San Diego, CA; clone RA3-6B2), CD19-PE/Dazzle594 (BioLegend, San Diego, CA; clone 6D5), CD3-AlexaFluor488 (BioLegend, clone 17A2), CD4-violetFluor450 (Tonbo Biosciences, clone GK1.5), CD8-PE/Cy7 (Tonbo Biosciences, clone 53-6.7), NK1.1-PE (Tonbo Biosciences, clone PK136), CD335/NKp46-APC (BioLegend, clone 29A1.4), CD11b-APC/Cy7 (Tonbo Biosciences, clone M1/70), CD11c-PerCp/Cy5.5 (Tonbo Biosciences, clone N418), Ly6G-PE (Tonbo Biosciences, clone RB6-8C5), and F4/80-FITC (Tonbo Biosciences, clone BM8.1,).

Techniques: Flow Cytometry, Two Tailed Test, IF-P